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biological source
Bacillus licheniformis
Quality Level
form
liquid
specific activity
≥0.75 Anson units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
activity assay: suitable
color
dark brown
application(s)
life science and biopharma
shipped in
ambient
storage temp.
2-8°C
General description
Alcalase® is a serine endopeptidase that consists primarily of subtilisin A.
Application
Alcalase® Enzyme, Bacillus licheniformis has been used in the proteolytic digestion and extraction of polysaccharides in various sponge species. It has also been used as an endoprotease enzyme in enzymatic hydrolysis for the production of fish protein hydrolysate.
Biochem/physiol Actions
Alcalase® is suitable for the hydrolysis of proteins with an optimal pH of 7.0-8.5. It is used as an additive in detergent, dehairing, and bating leather. Alcalase is also used in meat tenderizing, cheese flavor development, baked manufacture, and improving the digestibility of animal feeds. Commercial Alcalase® comprises a liquid enzymatic preparation containing 50% glycerol, 41% water, and 9% protease extract from Bacillus licheniformis.
Warning
Toxicity: Harmful (C)
Unit Definition
One unit is defined as the amount of enzyme that will release 1.0 µmol L-tyrosine from hemoglobin per min at 25°C, pH 7.5.
Reconstitution
Please refer to the vial label for lot-specific density.
Legal Information
Alcalase is a registered trademark of Novozymes Corp.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
监管及禁止进口产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Monitoring of sulfated polysaccharide content in marine sponges by Raman spectroscopy
Maia LF, et al.
Vibrational Spectroscopy, 87, 149-156 (2016)
Veymar G Tacias-Pascacio et al.
International journal of biological macromolecules, 165(Pt B), 2143-2196 (2020-10-23)
This review aims to cover the uses of the commercially available protease Alcalase in the production of biologically active peptides since 2010. Immobilization of Alcalase has also been reviewed, as immobilization of the enzyme may improve the final reaction design
Fish protein hydrolysate production by acid and enzymatic hydrolysis
Wisuthiphaet N, et al.
Applied Science and Engineering Progress, 9(4) (2016)
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