1.25182
Fractogel® EMD SO3⁻ (S) Resin
MiniChrom Column Fractogel® SO3 (S), volume 5 mL, prepacked column
Synonym(s):
MiniChrom Fractogel® SO3 (S) 5ml
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About This Item
UNSPSC Code:
41115711
ligand
(Sulfoisobutyl)
Quality Level
material
polypropylene housing
product line
Fractogel®
form
prepacked column
parameter
≤8 bar pressure
~80 cm/hr max. flow rate
column I.D. × L
8 mm × 100 mm
volume
5 mL
matrix active group
Methacrylate
mean particle size
20-40 μm
capacity
~150 mL, resin binding capacity
application(s)
recombinant protein
vaccine development
storage temp.
no temp limit
Related Categories
General description
8 x 100mm
Features and Benefits
MiniChrom Fractogel® SO3(S), 5 ml enables:
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (S) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- Efficient separation between monomer, dimer, and high molecular weight aggregates
- High binding capacity
- High resolution
- Efficient polishing step
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (S) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
Packaging
Plastic bag(s)
Legal Information
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
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