1.25085
Fractogel® EMD TMAE Hicap (M)
RoboColumn Fractogel® TMAE Hicap (M), volume 0.2 mL, prepacked column
Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
RoboColumn Fractogel® TMAE Hicap (M), 0.2 ml
UNSPSC Code:
41115711
ligand
(Trimethylammoniumethyl)
Quality Level
material
polypropylene housing
product line
Fractogel®
form
prepacked column
parameter
≤8 bar pressure
~180 cm/hr max. flow rate
column I.D. × L
5 mm × 10 mm
volume
0.2 mL
matrix active group
Methacrylate
mean particle size
40-90 μm
capacity
~180 mg, BSA binding capacity
application(s)
mAb
recombinant protein
vaccine development
separation technique
anion exchange
storage temp.
room temp
Related Categories
General description
5 x 10 mm
Features and Benefits
RoboColumn Fractogel® TMAE Hicap (M), 0.2 ml enables users to:
Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
- Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
Legal Information
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service