12-500
HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer
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About This Item
UNSPSC Code:
41105330
NACRES:
NA.42
eCl@ss:
32011807
biological source
human
form
liquid
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
shipped in
dry ice
Quality Level
General description
EGF stimulated HeLa cell lysate. The cell lysate was quantitated via BCA assay. This sell lysate demonstrated higher levels of Ras-GTP form present as compared to unstimulated HeLa cell lysates. The assay was performed using the Ras GTPase Activation ELISA Kit (Catalog # 17-424)
Biochem/physiol Actions
HeLa
Physical form
100 &micor;g of EGF stimulated HeLa cell lysate, in 40 µL of 1X MLB (Catalog 20-168) containing, 1 &micor;g/mL leupeptin, 1µg/mL aprotinin, 1 µg/mL pepstatin, 1mM PMSF.
Preparation Note
Stable for 6 months at -80C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. Use immediately in assay following thawing as Ras GTP form can rapidly hydrolyze to the inactive Ras GDP form.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Danger
hcodes
Hazard Classifications
ED ENV 1 - Eye Irrit. 2
Certificates of Analysis (COA)
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D V Marinkovic et al.
The Biochemical journal, 155(2), 209-216 (1976-05-01)
Aminoethylated beta-galactosidase from Escherichia coli was cleaved by CNBr. The fragment C4a was purified by gel filtration and ion-exchange chromatography. The molecular weight of the fragment C4a was determined to be 9000 +/- 600. The N-terminal amino acid was found
D V Marinkovic et al.
The Biochemical journal, 165(3), 417-423 (1977-09-01)
Carboxymethylated beta-galactosidase from Escherichia coli was dissociated at 100 degrees C to form carboxymethylated fragments A and B. The mol.wts. of carboxymethylated fragments A and B were determined by gel filtration to be 64300 and 22400 respectively. Sodium dodecyl sulphate/polyacrylamide-gel
Ioannis Partheniadis et al.
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Oregano essential oil (EO) enteric release powder was formulated by spray drying feed emulsions stabilized with polysaccharides (PSC) and Eudragit® L100 (PLM). Different modified starches were used in the PSC component. Spray-dried powders were evaluated for particle size and morphology
Brandon S Carpenter et al.
Journal of cell science, 128(5), 1034-1050 (2015-01-16)
GLI transport to the primary cilium and nucleus is required for proper Hedgehog (HH) signaling; however, the mechanisms that mediate these trafficking events are poorly understood. Kinesin-2 motor proteins regulate ciliary transport of cargo, yet their role in GLI protein
Christoph E Joosten et al.
Biotechnology progress, 19(3), 739-749 (2003-06-07)
Secreted human placental alkaline phosphatase (SEAP) was produced in a nonengineered Trichoplusia ni insect cell line, Tn-4s, using a recombinant Autographa californica baculovirus expression vector. The effect of culture conditions on SEAP specific yield and glycosylation was studied. When cultured
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