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Merck
CN

1.24546

Fluorescein-5-isothiocyanate (FITC)

for biochemistry and for fluorescence microscopy

Synonym(s):

Fluorescein-5-isothiocyanate (FITC)

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About This Item

Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
3326-32-7
Molecular Weight:
389.38
EC Index Number:
222-042-0
UNSPSC Code:
12171501
MDL number:
MFCD00005063

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InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

form

solid

composition

Dye, ≥95% spectrophotometric assay

mp

>360 °C

εmax

2200-2380 at 488-494 nm in 0.1 M NaOH at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

400

General description

In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.
FITC is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Sodium hydroxide solution 0.1 mol/l): 488 - 494 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380
TLC-Test: passes test

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品
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