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1.16845

Millipore

Deoxycholate agar

suitable for microbiology

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UNSPSC Code:
41106213
NACRES:
NA.74

form

plate
solid

Quality Level

200

packaging

pkg of 5 kg
pkg of 500 g

manufacturer/tradename

GranuCult®

storage condition

(Tightly closed. Dry)

technique(s)

microbiological culture: suitable

color

clear reddish to brown

pH

7.0-7.4 (25 °C, 45 g/L in H2O)

solubility

45 g/L

application(s)

industrial qc
microbiology

storage temp.

2-8°C

suitability

(enteric pathogens)
coliforms

Related Categories

General description

Deoxycholate agar contains peptone to provide nitrogen, vitamins, carbon, amino acids, and other nutrients to support the growth of microorganisms. Lactose is a fermentable carbohydrate. Sodium chloride and dipotassium phosphate maintain the osmotic balance. Sodium deoxycholate and ammonium iron citrate inhibit the growth of Gram-positive bacteria. Neutral red is a pH indicator. Agar acts as the solidifying agent.Fermentation of lactose is the basis for the differentiation of enteric bacteria. Lactose-positive bacteria, like coliform bacteria, produce acid from lactose and form red colonies. Lactose-negative bacteria, such as Salmonella spp. and Shigella spp., form colorless colonies.

Application

Deoxycholate Agar is a selective and differential plating medium for the isolation and differentiation of Gram-negative enteric bacteria.

Features and Benefits

  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
  • Excellent wettability, solubility, and free flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allowing many supplements to be included, with no need to add these separately

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Information

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Shigella spp. are predominantly connected to humans and are rarely isolated from other animals, with the exception of primates.

Cultivation of Shigella

Dehydrated culture media for cultivating Shigella come in granules that dissolve in water. Granulation of the media ensures homogenous distribution, which avoids clumping even in warm and humid conditions. 

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