1.16216
Peroxidase
(from horse radish) 10000 U/vial for biochemistry EC 1.11.1.7
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Synonym(s):
Peroxidase
Recommended Products
biological source
horseradish
Quality Level
form
solid
pH
6.5-7.5 (20 °C, 10 g/L in H2O)
storage temp.
2-8°C
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
General description
Horseradish peroxidase (HRP) is a glycoprotein that belongs to the class of oxidoreductase of the peroxidases family. It is found in horseradish roots. HRP contains significant amounts of carbohydrates and photohemin IX as its prosthetic group.
Biochem/physiol Actions
Peroxidases catalyze the cleavage of peroxides by redox reactions. The active center includes heme, cysteine thiols, selenium, manganese, and other chemical moieties. Peroxidase isoenzymes are essential for oxidizing reactive oxygen species, innate immunity, hormone biosynthesis, and the pathogenesis of many diseases. They are generally used for sensors, drug delivery devices, environment protection, bioremediation, and energy production. Horseradish peroxidase (HRP) is the widely used oxidoreductase in colorimetric, fluorimetric, chemiluminescent, electrochemical, and immunoassays. Its concentration can be measured with O-phenylenediamine (OPD) and hydrogen peroxide. HRP serves as a tracer for immunocytochemistry and is widely used in antibody-enzyme conjugates.
Features and Benefits
- Ideal for preparing small-sized antibody-enzyme conjugates
- Enables better cellular penetration through low-molecular-weight conjugates
- Small conjugate size ensures greater accessibility to antigenic structures within tissue sections
- Stable even under the conditions employed for crosslinking
Analysis Note
Activity
- Hydrogen peroxide and guajacol; pH 7.0; 25 °C:
about 170 U/mg
: ≥ 10000 U/Glas
Catalase: ≤ 1 U/mg
- Hydrogen peroxide and guajacol; pH 7.0; 25 °C:
about 170 U/mg
: ≥ 10000 U/Glas
Catalase: ≤ 1 U/mg
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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