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1.15939

Sigma-Aldrich

Nuclear fast red (C.I. 60760)

for microscopy Certistain®

Synonym(s):

Nuclear fast red (C.I. 60760)

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About This Item

Empirical Formula (Hill Notation):
C14H8NNaO7S
CAS Number:
6409-77-4
Molecular Weight:
357.27
MDL number:
MFCD00078493
UNSPSC Code:
41116130
EC Index Number:
229-088-0

Quality Level

400

form

solid

composition

Dye, ≥90% spectrophotometric assay

pH

5.5-7.0 (20 °C, 1 g/L in H2O)

solubility

1 g/L

bulk density

400‑450 kg/m3

εmax

380-422 at 557-559 nm in DMSO at 0.02 g/L
383-426 at 520-522 nm in DMSO at 0.02 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1

InChI key

IFSXZLJQEKGQAF-UHFFFAOYSA-M

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General description

Nuclear fast red (C.I. 60760) for microscopy Certistain®, is a dry dye for microscopy that is often used for staining nuclei in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of a Nuclear fast red staining solution. It is commonly used for counterstaining in histochemical reactions, e.g. in Berlin blue staining for detection of iron or in silver plating of reticulin fibers, as well as nuclear staining with Alcian blue. As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ 1 (Dimethyl sulfoxide): 557 - 559 nm
Absorption maximum λ 2 (Dimethyl sulfoxide): 520 - 522 nm
Spec. Absorptivity A 1%/1cm
- λ1max; 0.02 g/l; dimethyl sulphoxide:
380 - 422
- λ2max; 0.02 g/l; Dimethyl sulphoxide: 383 - 426
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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