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1.15936

Sigma-Aldrich

Erythrosine B (C.I. 45430)

for microscopy Certistain®

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Synonym(s):
Erythrosine B (C.I. 45430), Tetraiodofluorescein disodium salt
Empirical Formula (Hill Notation):
C20H6I4Na2O5
CAS Number:
16423-68-0
Molecular Weight:
879.86
MDL number:
MFCD00144257
UNSPSC Code:
41116130
EC Index Number:
240-474-8

Quality Level

400

form

solid

potency

1840 mg/kg LD50, oral (Rat)

composition

Dye, ≥80% gravimetric

pH

6-7 (20 °C, 10 g/L in H2O)

solubility

100 g/L

bulk density

470 kg/m3

εmax

930-1170 at 524-527 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C20H8I4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

IINNWAYUJNWZRM-UHFFFAOYSA-L

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General description

Erythrosin B (C.I. 45430)for microscopy Certistain®, is a dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Erythrosin B staining solutions. As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (gravimetrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 524 - 527 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 930 - 1170
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302 - H413

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(C)

182 °C - closed cup

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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