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1.15935

Sigma-Aldrich

Eosin Y (yellowish) (C.I. 45380)

for microscopy Certistain®

Synonym(s):

Eosin Y (yellowish) (C.I. 45380)

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About This Item

Empirical Formula (Hill Notation):
C20H6Br4Na2O5
CAS Number:
17372-87-1
Molecular Weight:
691.85
MDL number:
MFCD00005040
UNSPSC Code:
41116130
EC Index Number:
241-409-6

Quality Level

400

form

solid

potency

>2000 mg/kg LD50, oral (Rat)

composition

Dye, ≥85% spectrophotometric assay

pH

9.2 (20 °C, 10 g/L in H2O)

mp

295-296 °C

solubility

300 g/L

bulk density

710 kg/m3

εmax

1200-1400 at 515-518 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

SEACYXSIPDVVMV-UHFFFAOYSA-L

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General description

Eosin Y (C.I. 45380) is a Certistain® dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin Y staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances red-orange.
As the Eosin Y dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (gravimetrically): ≥ 85 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 515 - 518 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 1200 - 1400
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

WGK

WGK 3

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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