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1.15930

Sigma-Aldrich

Toluidine blue O (C.I.52040)

for microscopy Certistain®

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Synonym(s):
Toluidine blue O (C.I.52040), Toluidine blue O (C.I. 52040) Certistain®
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
92-31-9
Molecular Weight:
305.83
MDL number:
MFCD00011934
UNSPSC Code:
41116130
EC Index Number:
202-146-2

Quality Level

400

form

solid

composition

Dye, ≥85% spectrophotometric assay

pH

2.8 (25 °C in H2O)

solubility

30 g/L

bulk density

410 kg/m3

εmax

1330-1570 at 628-633 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C15H16N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H/q+1;/p-1

InChI key

HNONEKILPDHFOL-UHFFFAOYSA-M

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General description

Toluidine blue O (C.I. 52040) for microscopy Certistain® is adry dye that is used for the preparation of a staining solution for staining nuclei and for visualization of metachromasia in histological sections of human origin. As a Certistain® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (Diaminophenothiazins, spectrophotometrically): ≥ 85 %
Identity
- chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 628 - 633 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 1330 - 1570
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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