1.15338
Plate Count skimmed milk agar
GranuCult® prime, according to ISO 4833, suitable for microbiology, for aerobic bacteria
Synonym(s):
Casein-Peptone Dextrose Yeast Skimmed Milk Agar, Milk Plate Count Agar, Tryptone Glucose Yeast Skimmed Milk Agar
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About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
Agency
APHA
ISO 17410
ISO 6730 I IDF 101
ISO 8552 I IDF 132
according to ISO 4833
Quality Level
sterility
non-sterile
form
granular
packaging
pkg of 500 g
manufacturer/tradename
GranuCult® prime
storage condition
(Tightly closed. Dry.)
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
15-25°C
suitability
aerobic bacteria
Related Categories
General description
Plate Count skimmed milk agar is a non-selective medium for the enumeration of viable micro-organisms in milk and dairy products. The medium does not contain any inhibitors or indicators, and it is relatively rich in nutrients. The added skimmed milk is also free from inhibitory substances. The enzymatic digest of casein (tryptone) is a nitrogen source containing a high level of free amino acids, and yeast extract supplies the B-complex vitamins. Glucose provides an energy source for the growth of bacteria, while agar is the solidifying agent.
Application
Plate Count skimmed milk agar is suitable for the determination of the total microbial content from milk and dairy products.
Features and Benefits
- GranuCult® offers superior granulated culture media
- Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
- Excellent wettability, solubility, and free-flowing properties
- Convenient, with minimal component separation and clumping, even under warm or humid conditions
- High batch-to-batch reproducibility
- Prolonged shelf life of up to five years
- High number of test strains exceeding all regulatory demands
- Granulation technology allows many supplements to be included, with no need to add these separately
Analysis Note
Appearance (clearness): clear to opalescent
Appearance (colour): yellowish to yellowish-brown
pH-value (25 °C): 6.8 - 7.2
Solidification behaviour (2 hrs., 40 °C): liquid
Solidification behaviour (4 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)):
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003)):
Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 70 %
Recovery (Staphylococcus aureus ATCC 25923 (WDCM00034)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 70 %
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): ≥ 70 %
Incubation: 72 ± 3 hours at 30 ± 1 °C aerobic.
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar
Appearance (colour): yellowish to yellowish-brown
pH-value (25 °C): 6.8 - 7.2
Solidification behaviour (2 hrs., 40 °C): liquid
Solidification behaviour (4 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)):
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003)):
Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 70 %
Recovery (Staphylococcus aureus ATCC 25923 (WDCM00034)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 70 %
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): ≥ 70 %
Incubation: 72 ± 3 hours at 30 ± 1 °C aerobic.
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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