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Sigma-Aldrich

Ethidium bromide

(1% solution in water) for electrophoresis

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UNSPSC Code:
41105305
NACRES:
NA.51

Quality Level

form

liquid

pH

4.4 (20 °C in H2O)

density

1.00 g/cm3 at 20 °C

storage temp.

2-8°C

Application

Ethidium bromide has been used to stain polyacrylamide gels for small-scale transcription reactions. It has also been used for the quantification of DNA.

Biochem/physiol Actions

Ethidium bromide is a red fluorescent dye that reacts with the DNA of membrane-damaged cells by intercalating. It is used for visualizing DNA in gels. Ethidium bromide is carcinogenic with moderate toxicity.

Features and Benefits

Robust and easy to use

Analysis Note

Identity (UV/VIS-Spectrum): passes test
Absorption maximum (water): 478 - 482 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.004 %; water): 140 - 160
Suitability for electrophoresis: passes test

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Inhalation - Muta. 2

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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