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Millipore

GranuCult® prime MRS agar (de MAN, ROGOSA and SHARPE)

according to ISO 15214, for Lactobacillus spp., suitable for microbiology

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Synonym(s):
Lactobacillus Agar, Lactobacillus Agar according to DeMan, Rogosa and Sharpe, Lactobacillus selective medium, DeMan, Rogosa, Sharpe agar
UNSPSC Code:
41106200
NACRES:
NA.74

Agency

APHA
according to ISO 15214

Quality Level

200

form

medium granules (dehydrated (DCM))

packaging

pkg of 500 g

manufacturer/tradename

GranuCult® prime

storage condition

dry media (Keep tightly closed)

technique(s)

microbiological culture: suitable

color

beige

pH

5.5-5.9 (25 °C, 66.24 g/L in H2O, after autoclaving)

solubility

68.2 g/L

bulk density

600 kg/m3

application(s)

food and beverages
microbiology

storage temp.

2-8°C

suitability

Lactobacillus spp.

Related Categories

General description

GranuCult® prime MRS agar (de MAN, ROGOSA and SHARPE) is a Lactobacillus selective medium. This medium contains polysorbate, acetate, magnesium, and manganese, which act as special growth factors for Lactobacilli, as well as a rich nutrient base. As the medium exhibits a low degree of selectivity, it may support the growth of Pediococcus and Leuconostoc species and other secondary bacteria.

Application

GranuCult® prime MRS agar is suitable for isolation, enumeration, and cultivation of Lactobacillus spp. and other mesophilic lactic acid bacteria from all types of materials.

Features and Benefits

  • GranuCult® offers superior granulated culture media.
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions.
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): brown
pH-value (25 °C): 5.6 - 5.8
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)):
Inoculum on reference medium (Lactobacillus sakei ATCC 15521 (WDCM 00015)):
Inoculum on reference medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)):
Inoculum on reference medium (Pediococcus pentosaceus ATCC 33316 (WDCM 00158)):
Inoculum on reference medium (Pediococcus damnosus ATCC 29358 (WDCM 00022)):
Colony count (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)):
Colony count (Lactobacillus sakei ATCC 15521 (WDCM 00015)):
Colony count (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)):
Colony count (Pediococcus pentosaceus ATCC 33316 (WDCM 00158)):
Colony count (Pediococcus damnosus ATCC 29358 (WDCM 00022)):
Recovery on test medium (Lactobacillus sakei ATCC 15521 (WDCM 00015)): ≥ 70 %
Recovery on test medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)): ≥ 70 %
Recovery on test medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)): ≥ 70 %
Recovery on test medium (Pediococcus pentosaceus ATCC 33316 (WDCM 00158)): ≥ 70 %
Recovery on test medium (Pediococcus damnosus ATCC 29358 (WDCM 00022)): ≥ 70 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): total inhibition
Growth (Bifidobacterium bifidum ATCC 11863): good growth
Incubation: 72 ± 3 hours at 30 ± 1 °C aerobic; Bifidobacterium anaerobic
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: MRS-Agar

Other Notes

  • Do not use clumped or discolored medium.
  • The prepared medium is clear and brown.
  • Prepared plates can be stored at +2 °C to + 8 °C °C in the dark and protected against evaporation for up to 14 days.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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