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1.10316

Millipore

Fractogel® EMD TMAE Hicap (M)

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Synonym(s):
Biochromatography, Fractogel® Ion exchangers, Ion exchanger for LC (Fractogel®TSK/EMD), Fractogel® EMD TMAE Hicap (M)
UNSPSC Code:
41115711

ligand

(Trimethylammoniumethyl)

Quality Level

400

description

strong anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)

sterility

sterile (Caustic Stable)

form

resin

specific analyte(s)

MAbs

parameter

180 cm/hr flow rate
8 bar max. pressure

matrix active group

methacrylate

mean particle size

40-90 μm

capacity

180 mg binding capacity (BSA/mg of resin)

transition temp

flash point 35 °C (calculated)

density

1.430 g/cm3 at 20 °C

bulk density

1000 kg/m3

application(s)

recombinant protein

separation technique

anion exchange
strong anion exchange

storage temp.

2-30°C

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General description

Strong anion exchange Fractogel® resin featuring the tentacle technology with a trimethylammoniumethyl functional group for purification of acidic and neutral proteins and peptides from multiple sources, DNA and virus removal, plasmid purification, vaccine purification, and more

Features and Benefits

Fractogel® EMD TMAE Hicap (M) enables users to:

  • Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
  • Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

Packaging

  • 1.10316.0100: Fractogel® EMD TMAE Hicap (M) Resin 100ml
  • 1.10316.0010: Fractogel® EMD TMAE Hicap (M) Resin 10ml
  • 1.10316.0500: Fractogel® EMD TMAE Hicap (M) Resin 500ml
  • 1.10316.5000: Fractogel® EMD TMAE Hicap (M) Resin 5L

Plastic bottle

Analysis Note

Appearance: Milky turbid suspension, free from impurities (foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.03 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (bovine serum albumin): 160 - 200 mg/ml
Functional test (b:a): ≤ 0.25
Functional test: Separation of conalbumin and human serum albumin

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

95.0 °F

Flash Point(C)

35 °C

Certificates of Analysis (COA)

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