1.10282
OF basal medium
for the differentiation and classification of gram-negative intestinal bacteria
Synonym(s):
OF test medium, Oxidation-fermentation test medium acc. to HUGH and LEIFSON
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About This Item
UNSPSC Code:
41106213
Quality Level
form
powder (dehydrated (DCM))
technique(s)
microbiological culture: suitable
pH
7.0-7.2 (37 °C, 12 g/L in H2O, after autoclaving)
solubility
12 g/L
bulk density
770 kg/m3
application(s)
clinical
microbiology
storage temp.
15-25°C
suitability
enterobacteriaceae
General description
for the differentiation and classification of gram-negative intestinal bacteria
Analysis Note
Appearance (clearness)clearAppearance (colour)dark green to blue-greenpH-value (25 °C)6.9 - 7.3Growth aerobic (Escherichia coli ATCC 25922 (WDCM 00013))good to very goodGrowth aerobic (Staphylococcus aureus ATCC 25923 (WDCM 00034))good to very goodGrowth aerobic (Kocuria rhizophila ATCC 9341)good to very goodGrowth aerobic (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))good to very goodGrowth aerobic (Alcaligenes faecalis ATCC 19209)good to very goodGrowth aerobic (Pseudomonas alcaligenes ATCC 14909)good to very goodColour change to yellow (Escherichia coli ATCC 25922 (WDCM 00013))+Colour change to yellow (Staphylococcus aureus ATCC 25923 (WDCM 00034))+Colour change to yellow (Kocuria rhizophila ATCC 9341)+Colour change to yellow (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))+Colour change to yellow (Alcaligenes faecalis ATCC 19209)-Colour change to yellow (Pseudomonas alcaligenes ATCC 14909)-Colour change to yellow anaerobic (Escherichia coli ATCC 25922 (WDCM 00013))+Colour change to yellow anaerobic (Staphylococcus aureus ATCC 25923 (WDCM 00034))+Colour change to yellow anaerobic (Kocuria rhizophila ATCC 9341)(-)Colour change to yellow anaerobic (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))-Colour change to yellow anaerobic (Alcaligenes faecalis ATCC 19209)-Colour change to yellow anaerobic (Pseudomonas alcaligenes ATCC 14909)-Incubation: E.coli ATCC 25922 and Pseud.aerug ATCC 27853 24 hrs; 37 °C; aerobic; other strains 48 hrs.; 35 °C; aerobic.
Tested with D(+)glucose.
Tested with D(+)glucose.
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Regulatory Information
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