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1.08178

Supelco

Trifluoroacetic acid

for protein sequence analysis

Synonym(s):

TFA

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1 MG
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CN¥25,615.51

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0.2 MG
CN¥7,657.47
1 MG
CN¥15,961.97
5 X 1 MG
CN¥25,615.51

About This Item

Linear Formula:
CF3COOH
CAS Number:
76-05-1
Molecular Weight:
114.02
Beilstein:
742035
MDL number:
MFCD00004169
UNSPSC Code:
12352106
EC Index Number:
200-929-3
NACRES:
NA.21

CN¥7,657.47

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vapor density

3.9 (vs air)

Quality Level

200

vapor pressure

97.5 mmHg ( 20 °C)

Assay

≥99.7% (acidimetric)

form

liquid

impurities

≤0.02% Water (Karl Fischer)

refractive index

n20/D 1.3 (lit.)

pH

1 (10 g/L in H2O)

bp

72.4 °C (lit.)

mp

−15.4 °C (lit.)

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clone

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clone

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clone

M2, monoclonal

clone

M2, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

FITC conjugate

conjugate

CY3 conjugate

conjugate

biotin conjugate

conjugate

unconjugated

antibody form

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antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

form

buffered aqueous solution

form

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

form

buffered aqueous glycerol solution

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Application

Trifluoroacetic acid (TFA) can be used as an ion-pairing agent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and can be easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).[1]

Analysis Note

Assay (acidimetric): ≥ 99.7 %
Identity (IR-spectrum): passes test
Density (d 20 °C/20 °C): about 1.53
Boiling point: about 72 °C
Water (according to Karl Fischer): ≤ 0.02 %

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H314 - H332 - H412

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

212.0 °F - Pensky-Martens closed cup

Flash Point(C)

> 100 °C - Pensky-Martens closed cup

Regulatory Information

监管及禁止进口产品
  • Specification Sheet

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    Y Chen et al.
    Journal of chromatography. A, 1043(1), 9-18 (2004-08-20)
    Trifluoroacetic acid (TFA) remains the dominant mobile phase additive for reversed-phase high-performance liquid chromatography (RP-HPLC) of peptides after more than two decades since its introduction to this field. Generally, TFA has been employed in a concentration range of 0.05-0.1% (6.5-13

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