TLC plates, Silica gel 60 RP-2 F₂₅₄ (silanized) 25 Glass plates 20 x 20 cm

Glass TLC plates, RP-2 modified silica gel coated with flourescent indicator F254. The RP-2 modified silica gel TLC plates come in a quantity of 25 size 20x20 cm for thin layer chromatography applications.

RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.

Replaces: 5747-7; 5747

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 9.5 µm - 11.5 µm
Layer thickness: 190 - 250 µm Deviation of layer thickness per plate: ≤ 40 µm
Chromatographic testings:
A) Cholesterin test
hRf-values - cholesterin, cholesterin test: 80 - 90
B) Steroid test - methyltestosterone,
steroid test: 35 - 44 - Reichstein S,
steroid test: 46 - 54 - hydrocortisone,
steroid test: 53 - 61
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) aceton/ water (60/40 v/v)