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1.05715

Supelco

TLC plates, Silica gel 60 F₂₅₄

pkg of 25 plates, plate L × W 20 cm × 20 cm, glass support

Synonym(s):

Thin Layer Chromatography Silica Plates

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About This Item

UNSPSC Code:
41115711
NACRES:
NB.21

material

glass support
silica gel 60 matrix

Quality Level

feature

binder Organic Polymer
fluorescent indicator

packaging

pkg of 25 plates

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

250 μm

plate L × W

20 cm × 20 cm

particle size

10-12 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

General description

25 Glass plates 20 x 20 cm

Linkage

Replaces: 5715-7; 5715

Analysis Note

Specific surface area (according to BET; 5-Pt.measurement): 480 - 540 m²/g
Pore volume(N₂-isotherm):0.74 - 0.84 ml/g
d 50 (laserdiffraction, size distribution): 9.7 - 11.7 µm
Layerthickness: 210 - 270 µm
Deviationof layer thickness per plate: ≤ 35 µm
Chromatographic testing:
A) colourtest
hRf-values
- bleu viforganol, colour test, lipophile: 11 - 25
- CeresBlack G, colour test, lipophile: 34 - 48
- ceresViolet brn, colour test, lipophile: 52 - 67
separationnumber (colour test, lipophile): ≥ 10.5
B)Steroid test
hRf-values
- Hydrocortisone, steroid test: 25 - 37
- Reichstein S, steroid test: 37 - 49
- Methyltestosterone, steroid test: 42 - 54
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel. humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel. humidity)

Certificates of Analysis (COA)

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Articles

Thin Layer Chromatography Troubleshooting: This article details TLC tips and tricks that one must consider while performing different steps of the technique.

HPTLC plates provide superior separation, speed, and sensitivity, making them ideal for advanced qualitative and quantitative analysis.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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