1.05642
HPTLC plates, Silica gel 60 F₂₅₄
pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support
Synonym(s):
HPTLC Silica Gel Plates
About This Item
material
glass support
silica gel 60 matrix
Quality Level
feature
binder Organic Polymer
fluorescent indicator
packaging
pkg of 50 plates
technique(s)
thin layer chromatography (TLC): suitable
layer thickness
200 μm
plate L × W
20 cm × 10 cm
particle size
5-6 μm
pore size
60 Å medium pore diameter
storage temp.
2-30°C
Related Categories
General description
Application
Linkage
Analysis Note
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)
Certificates of Analysis (COA)
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Articles
Learn about our recently launched HPTLC calibration mix, developed in collaboration with CAMAG, that enables for an efficient and reliable universal system suitability testing (SST).
An HPTLC method for rapid urea detection in milk using a TLC plate scanner and Silica Gel 60 F254 HPTLC plate.
Development of an HPTLC based method, using Silica Gel 60 F254 plate, to determine Betamethasone Sodium Phosphate over a concentration range of 1 to 7 µg/spot.
In this article we present several HPTLC applications and analytical standards for ginsenosides.
Protocols
HPTLC was used to analyze caffeine in coffee. Filtration of coffee through a PTFE syringe filter was the only sample preparation. Quantitation was conducted with a TLC scanner.
In the present study we demonstrate compliance of silica gel 60 F254 HPTLC plates with USP requirements, and how HPTLC plates are suitable for the Sildenafil citrate-Limit of Imidazole test.
Powdered black pepper tested for pierine content follows USP guidelines using HPTLC and HPLC for dietary supplements.
HPTLC fingerprinting enables rapid identification of passion flower with related reference materials.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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