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Millipore

GranuCult® Mannitol salt phenol-red agar

USP, EP, JP, suitable for microbiology

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Synonym(s):
Mannitol salt agar, MSA, Chapman Agar
UNSPSC Code:
41106213
NACRES:
NA.74

Agency

EP
JP
USP

Quality Level

200

form

medium granules

feature

ready-to-use

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

7.2-7.6 (25 °C, 108 g/L in H2O, after autoclaving)

solubility

108 g/L

bulk density

770 kg/m3

application(s)

bioburden testing
pharmaceutical
microbiology

storage temp.

15-25°C

suitability

Staphylococcus spp. (pathogenic)

Related Categories

General description

Mannitol salt phenol-red agar is a modified version of the selective agar for the isolation and presumptive identification of Staphylococcus aureus. The composition of Mannitol Salt Agar supports the growth of Staphylococcus aureus, whereas the high salt content of 7.5 % inhibit many other microorganisms. Degradation of mannitol to acid correlates with the pathogenicity of S. aureus and thus, serves as an indicator for this species. Some halophilic Enterococci and Vibriones also grow on Mannitol Salt Agar. In contrast to the most other Staphylococci, S. aureus can build acids from mannitol. Therefore, they build up yellow colonies with yellow zones. S. capitis, S. simulans, S. carnosus, S. scuri, S. lentus, and S. gallinarum are also mannitol positive.

Application

Mannitol salt phenol-red agar is used for the isolation and presumptive identification of Staphylococcus aureus in non sterile pharmaceuticals and food.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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