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Merck
CN

1.04194

Fast green FCF (C.I. 42053)

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About This Item

Empirical Formula (Hill Notation):
C37H34N2Na2O10S3
CAS Number:
2353-45-9
Molecular Weight:
808.85
NACRES:
NA.21
MDL number:
MFCD00013053

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InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3-,40,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

InChI key

XJBPDZVCYTYRIN-UHFFFAOYSA-L

form

solid

potency

>2000 mg/kg LD50, oral (Rat)

composition

Dye, ≥85% spectrophotometric assay

pH

2.7 (20 °C, 10 g/L in H2O)

mp

290 °C (decomposition)

solubility

150 g/L

bulk density

350 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

Quality Level

200

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Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Analysis Note

Dye content (spectrophotometrically): ≥ 85 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 622 - 626 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 1360 - 1610
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Application

for microscopy Certistain®

General description

The dry dye Fast Green FCF (C.I. 42053) for microscopy Certistain®, is used for the preparation of a staining solution that can be used for modified connective tissue staining acc. to van Gieson in histological sections. Further it is applied in histology as counterstaining in e.g. trichrome staining acc. to Masson and additionally for histochemical staining, as well as in Cytology as counterstain instead of light green SF in Papanicolaou′s staining. As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.

pictograms

Exclamation markHealth hazard
GHS07,GHS08

signalword

Warning

Hazard Classifications

Muta. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

352.4 °F - closed cup

flash_point_c

178 °C - closed cup

Regulatory Information

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