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1.04190

Sigma-Aldrich

Fuchsin acid (C.I. 42685)

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Empirical Formula (Hill Notation):
C20H17N3Na2O9S3
CAS Number:
3244-88-0
Molecular Weight:
585.54
UNSPSC Code:
12352106
NACRES:
NA.21

form

solid

Quality Level

200

composition

Dye, ≥60% spectrophotometric assay

pH

3-4 (20 °C, 10 g/L in H2O)

mp

>130 °C (Decomposition)

solubility

10 g/L

bulk density

920 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,23H,21-22H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2*+1/p-2

InChI key

WZRZTHMJPHPAMU-UHFFFAOYSA-L

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Application

Fuchsin acid (C.I. 42685) for microscopy Certistain®, is a triarylmethane dye used, among others, in trichrome connective tissue stainings acc. to Mallory and van Gieson. Mallory developed a staining procedure for visualization of collagenous connective tissue, which has been modified and improved over the years.It enables a good differentiation of individual tissue components, provided that the tissue has been fixed in Zenker’s solution. As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.
for microscopy Certistain®

Analysis Note

Dye content (spectrophotometrically): ≥ 60 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Hydrochloric acid 0.0025 mol/l): 540 - 549 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; hydrochloric acid 0.0025 mol/l): 780 - 1310
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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