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Merck
CN

1.04172

Toluidine blue O (C.I.52040)

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About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
92-31-9
Molecular Weight:
305.83
UNSPSC Code:
41116130
NACRES:
NA.21
MDL number:
MFCD00011934

Key Documents

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SMILES string

[s+]1c2c(nc3c1cc(cc3)N(C)C)cc(c(c2)N)C.[Cl-]

InChI

1S/C15H16N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H/q+1;/p-1

InChI key

HNONEKILPDHFOL-UHFFFAOYSA-M

form

solid ()

composition

Dye, ≥85% spectrophotometric assay

pH

2.8 (25 °C in H2O)

solubility

30 g/L

bulk density

410 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

Quality Level

200

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Analysis Note

Dye content (Diaminophenothiazins, spectrophotometrically): ≥ 85 %
Identity
- chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 628 - 633 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 1330 - 1570
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Application

for microscopy Certistain®

General description

Toluidine blue O (C.I. 52040) for microscopy Certistain® is adry dye that is used for the preparation of a staining solution for staining nuclei and for visualization of metachromasia. As a CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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