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1.04141

Millipore

Singlepath® E. coli O157 Kit

suitable for immunological and lateral flow assay in Food and Beverages applications

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Synonym(s):
Rapid E. Coli Food Test
UNSPSC Code:
41116129
NACRES:
NA.24

product name

Singlepath® E. coli, suitable for lateral flow assay, for Escherichia coli, input: food(s)

Agency

according to AOAC® PTM

Quality Level

200

product line

Singlepath®

shelf life

24 mo.

packaging

pack of 25 strips

manufacturer/tradename

Singlepath®

technique(s)

lateral flow assay: suitable
microbe id | immunological: suitable

input

food(s)

application(s)

food and beverages
pathogen testing

storage temp.

2-8°C

suitability

Escherichia coli (O157)
enterovirulent E. coli (EEC)

Related Categories

General description

Singlepath® E. coli is a rapid diagnostic test, designed to detect E. coli bacteria in various food and environmental samples. The Singlepath® E. coli is an immunochromatographic rapid test based on gold-labeled antibodies to detect the presence of a specific antigen, in this case, the E. coli bacteria. This lateral flow test is based on the immune flow principle and is a convenient and simple-to-use, one-step device. The test includes a nitrocellulose membrane strip that is coated with antibodies specific to E. coli antigens. When the sample is applied to the test strip, any E. coli antigens present will bind to the antibodies on the strip, resulting in the development of a visible test line. The test takes approximately 20 minutes to complete, and the results are interpreted based on the presence or absence of the test line. The detection limit can be regarded as less than one colony-forming unit of E. coli O157 (including H7) in a 25 g food sample, depending on the serogroup. No additional equipment expenditure is needed.

Application

SINGLEPATH® E. coli O157 is used for pathogen detection in industrial microbiology. This kit is designed for testing food and environmental samples. The test is intended to be used in food-analyzing laboratories for the presumptive qualitative detection of E. coli O157 (including H7) from a variety of foods. The test has been validated and approved for use in raw ground beef and pasteurized whole milk.

Features and Benefits

  • Rapid and reliable method
  • High sensitivity and specificity compared to conventional culture methods
  • Can be performed without the need for specialized equipment or personnel.

Linkage

Replaces: 1.04141.0002

Analysis Note

Performance test
- Escherichia coli O157:H7 ATCC 35150:
passes test
- E. coli O157:H7 HUSCL8: passes test
- E. coli O55:H7 TB182A: passes test

Legal Information

AOAC is a registered trademark of AOAC International
SINGLEPATH is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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