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Key Documents

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Safety Information

1.04122

Sigma-Aldrich

Methylene blue (C.I.52015)

Synonym(s):

Methylene Blue trihydrate

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S · 3H2O
CAS Number:
7220-79-3
Molecular Weight:
373.90
Beilstein:
3599847
MDL number:
MFCD00012111
UNSPSC Code:
41116130
Pricing and availability is not currently available.

form

solid

Quality Level

200

potency

1180 mg/kg LD50, oral (Rat)

composition

Dye, ≥82% spectrophotometric assay

pH

3 (20 °C, 10 g/L in H2O)

mp

190 °C (dec.) (lit.)

solubility

50 g/L

density

1.757 g/cm3 at 22 °C

bulk density

400‑600 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

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1 of 4

This Item
1159436210OPM9140
亚甲蓝 三水合物

104122

亚甲蓝 三水合物

亚甲蓝（C.I.52015） for microscopy Certistain®

115943

亚甲蓝（C.I.52015）

亚甲蓝 三水合物 OmniPur® Grade

6210OP

亚甲蓝 三水合物

亚甲蓝 certified by the BSC

M9140

亚甲蓝

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

solubility

50 g/L

solubility

50 g/L

solubility

-

solubility

H2O: 4mg/4ml

form

solid

form

solid

form

powder

form

powder

composition

Dye, ≥82% spectrophotometric assay

composition

Dye, ≥82% spectrophotometric assay

composition

-

composition

Dye content, ≥82%

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

-

storage temp.

room temp

Quality Level

200

Quality Level

400

Quality Level

100

Quality Level

200

General description

Methylene blue (C.I. 52015) for microscopy Certistain® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. As a CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain®

Analysis Note

Dye content (spectrophotometrically): ≥ 82 %
Identity
- Chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 660 - 665 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 2250 - 2750
TLC-Test: passes test
Loss on drying (110 °C): 10 - 15 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Regulatory Information

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