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1.02896

Millipore

LEIFSON Agar

Deoxycholate citrate agar acc. to LEIFSON, modified for microbiology

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About This Item

UNSPSC Code:
41106213

Quality Level

200

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

7.4-7.6 (37 °C, 48 g/L in H2O, after autoclaving)

solubility

47.5 g/L

bulk density

690 kg/m3

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Salmonella spp.
Shigella spp.

Analysis Note

Appearance (clearness): clear
Appearance (colour): reddish-brown
pH-value (25 °C): 7.3 - 7.7
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): poor to fair
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Growth (Shigella flexneri ATCC 12022 (WDCM 00126)): fair to very good
Growth (Shigella sonnei ATCC 11060): fair to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis NCTC 5188 (WDCM 00119)): good to very good
Growth (Proteus mirabilis ATCC 14273): good to very good
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): none
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): none
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): pink-red
Colony colour (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): pink-red
Colony colour (Shigella flexneri ATCC 12022 (WDCM 00126)): colourless
Colony colour (Shigella sonnei ATCC 11060): colourless
Colony colour (Salmonella typhimurium ATCC 14028 (WDCM 00031)): colourless
Colony colour (Salmonella enteritidis NCTC 5188 (WDCM 00119)): colourless
Colony colour (Proteus mirabilis ATCC 14273): colourless to brownish
Black centre (Salmonella typhimurium ATCC 14028 (WDCM 00031)): +
Black centre (Salmonella enteritidis NCTC 5188 (WDCM 00119)): +
Black centre (Proteus mirabilis ATCC 14273): +/-
Incubation: 24 hrs.; 35 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

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Articles

Cultivation of Shigella

Shigella spp. are predominantly connected to humans and are rarely isolated from other animals, with the exception of primates.

Cultivation of Shigella

Dehydrated culture media for cultivating Shigella come in granules that dissolve in water. Granulation of the media ensures homogenous distribution, which avoids clumping even in warm and humid conditions. 

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