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Key Documents

1.02129

Supelco

Chromolith® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

Synonym(s):

Chromolith® Performance RP-18 endcapped 100-4.6 HPLC column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

Pricing and availability is not currently available.

Product Name

Chromolith® RP-18 endcapped, L × I.D. 100 mm x 4.6 mm HPLC column

material

PEEK column

Quality Level

Agency

suitable for USP L1

product line

Chromolith®

feature

endcapped

packaging

1 ea of

extent of labeling

18% Carbon loading

parameter

200 bar pressure
45 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

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This Item
726226922274549
application(s)

cleaning products
cosmetics
environmental
food and beverages
personal care

application(s)

environmental
food and beverages

application(s)

environmental
food and beverages

application(s)

environmental
food and beverages

format

single component solution

format

single component solution

format

single component solution

format

single component solution

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

concentration

1000 mg/L lactate in water (nominal concentration)

concentration

1000 mg/L in water (nominal concentration)

concentration

1000 mg/L in water (nominal concentration)

concentration

1000 mg/L gluconate in water (nominal concentration)

General description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® Performance RP-18 endcapped HPLC columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å) and the surface modification is octadecylsilane with full end-capping. Chromolith® Performance RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column.

Chromolith® Performance RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
  • Rapid separations at very low column back-pressure
  • High matrix tolerance, allowing time and cost savings with simplified workflows
  • Cost savings due to extended column lifetimes
  • Connection of columns, flow gradients and the use of mobile phases with higher viscosity


The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032] Or Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451] with the Chromolith® Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]

The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Need more efficiency?
Chromolith® HighResolution RP-18 endcapped 100-4.6 HPLC columns [1.52022] provide a column efficiency exceeding 140,000 plates/meter.

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Application


  • Salt pollution reduces turbidity, dissolved organic matter, and cyanobacteria in experimental vernal pool communities: Discusses environmental applications of calcium chloride, focusing on its use in managing ecosystems affected by salt pollution, demonstrating its role in environmental remediation and management. (Vigil and Schuler, 2024).



Analysis Note

Appearance: conforms
Carbon content: 17,0 - 18,6 %
Pressure: ≤ 30 bar
stability testing: conforms
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3,9 - 4,6
Tailing (USP): 0,90 - 1,60 %
Selectivity
separation factor (anthracene/progesterone): 1,95 - 2,35

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Storage Class Code

10-13 - German Storage Class 10 to 13


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    Articles

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    LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

    Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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