1.01800
Alkaline Peptone Water
According to ISO 8914, FDA-BAM, APHA, and AOAC; For microbiology
Synonym(s):
APW; Peptone Water, alkaline; Peptone Water
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About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
Agency
AOAC®
APHA
ISO 8914
Quality Level
reg. compliance
FDA BAM
form
medium granules (dehydrated (DCM))
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
8.3-8.7 (25 °C, 20 g/L in H2O, after autoclaving)
solubility
20 g/L
application(s)
food and beverages
microbiology
storage temp.
15-25°C
suitability
Vibrio spp.
Related Categories
General description
Alkaline Peptone Water is recommended for the enrichment and isolation of Vibrio cholerae and Vibrio species. The growth of a broad spectrum of Vibrio sp. is promoted by peptones, a sodium chloride concentration of 10 g/liter, and a high pH of 8.5. The alkaline pH of this medium allows the growth of Vibrio species, while it inhibits the growth of other unwanted flora.
Application
Alkaline Peptone Water is used for the enrichment of Vibrio cholerae and Vibrio sp. in food and other materials.
Analysis Note
Appearance (clearness): clear
Appearance (colour): yellow
pH-value (25 °C): 8.3 - 8.7
Colony count (Vibrio vulnificus ATCC 33149): ≥ 1E+06 CFU/ml
Colony count (Vibrio cholerae Eltor Inaba CH 38): ≥ 1E+06 CFU/ml
Colony count (Vibrio cholerae Eltor Ogawa CH 60): ≥ 1E+06 CFU/ml
Colony count (Vibrio parahaemolyticus ATCC 17802 (WDCM 00037)): ≥ 1E+06 CFU/ml
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: bloog agar.
Appearance (colour): yellow
pH-value (25 °C): 8.3 - 8.7
Colony count (Vibrio vulnificus ATCC 33149): ≥ 1E+06 CFU/ml
Colony count (Vibrio cholerae Eltor Inaba CH 38): ≥ 1E+06 CFU/ml
Colony count (Vibrio cholerae Eltor Ogawa CH 60): ≥ 1E+06 CFU/ml
Colony count (Vibrio parahaemolyticus ATCC 17802 (WDCM 00037)): ≥ 1E+06 CFU/ml
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: bloog agar.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
AOAC is a registered trademark of AOAC International
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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