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1.01398

Sigma-Aldrich

Malachite green oxalate (C.I.42000)

for microscopy and for microbiology

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Synonym(s):
Malachite green oxalate (C.I.42000)
Empirical Formula (Hill Notation):
C52H54N4O12
CAS Number:
2437-29-8
Molecular Weight:
927.00
UNSPSC Code:
41116130
EC Index Number:
219-441-7

Quality Level

400

form

solid

composition

Dye, ≥90% spectrophotometric assay

pH

2.4 (24 °C, 10 g/L in H2O)

mp

159 °C

solubility

110 g/L

bulk density

400‑500 kg/m3

εmax

1730-1960 at 616-620 nm in water at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/2C23H25N2.3C2H2O4/c2*1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3*3-1(4)2(5)6/h2*5-17H,1-4H3;3*(H,3,4)(H,5,6)/q2*+1;;;/p-2

InChI key

CNYGFPPAGUCRIC-UHFFFAOYSA-L

General description

Malachite green Oxalate (C.I. 42000) is a dry dye that is used for the preparation of a staining solution that can be used for a spore staining according to Rakette. It is possible to use Malachite green dye for differential spore staining in bacteriological samples of human origin, Dye is used in a hot staining method to stain specifically spores. Counterstain with a red dye for better visualization. The dye has a constant dye content and a reliable high quality and thus, is registered and certified as IVD & CE product, one of the major precondition for a reproducible diagnostic staining.

Analysis Note

Dye content (spectrophotometrically): ≥ 90% (als C₄₆H₅₀N₄ *2 C₂HO₄ *C₂H₂O₄)
Identity
- oxalate:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 616 - 620 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; water): 1730 - 1960
TLC-Test: passes test
Loss on drying (50 °C / <1 hPa): ≤ 7%
Suitability for microbiology: passes test
Suitability for microscopy: passes test

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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