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Merck
CN

1.00211

Carminic acid (C.I. 75470)

GR for analysis and for microscopy

Synonym(s):

Carminic acid (C.I. 75470)

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About This Item

Empirical Formula (Hill Notation):
C22H20O13
CAS Number:
1260-17-9
Molecular Weight:
492.39
EC Index Number:
215-023-3
UNSPSC Code:
41116130
MDL number:
MFCD00167028

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InChI

1S/C22H20O13/c1-4-8-5(2-6(24)9(4)22(33)34)13(25)10-11(15(8)27)16(28)12(18(30)17(10)29)21-20(32)19(31)14(26)7(3-23)35-21/h2,7,14,19-21,23-24,26,28-32H,3H2,1H3,(H,33,34)/t7-,14-,19+,20-,21-/m1/s1

InChI key

DGQLVPJVXFOQEV-NGOCYOHBSA-N

assay

≥92%

form

solid

pH

1.6 (20 °C, 10 g/L in H2O)

mp

136 °C (decomposition)

solubility

30 g/L

bulk density

490 kg/m3

εmax

160-187 at 492-495 nm in hydrochloric acid at 0.014 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

Quality Level

400

General description

The dye Carminic acid (C.I. 75470) GR for analysis and for microscopy, is a dry dye used to prepare dye-solutions. Together with a mordant solution of lithium, aluminium or boron it is used as a lake for staining. Carminic acid is applied for various stainings, e.g. for the detection of glycogen and mucous substances (Best′s carmine), for nucelar staining or for vital staining. The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

Carminic acid (C.I. 75470) For staining nuclei in histological sections. Used to prepare staining solutions.
CAS 1260-17-9, pH 1.6 (10 g/l, H₂O, 20 °C).

Analysis Note

Assay (spectrophotometrically; calculated on dried substance)≥ 92 %Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (Hydrochloric acid 0.06 mol/l)492 - 495 nmSpec. Absorptivity A 1%/1cm (λmax; 0.014 g/l; Hydrochloric acid 0.06 mol/l; calc. on dried substance)160 - 187Loss on drying (110 °C)≤ 10 %Suitability for microscopypasses test

Regulatory Information

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