All Photos(2)
Synonym(s):
Kruppel-like factor 2 (lung), Lung krueppel-like factor, krueppel-like factor 2, lung Kruppel-like zinc finger transcription factor, Kruppel-like factor LKLF
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
technique(s)
ChIP: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Klf2(16598)
General description
Krueppel-like factor 2 (Klf2) is a novel protein belonging to the KLF family of transcription factors, a subfamily of approximately 20 members characterized by their possession of a zinc-finger domain and functioning as transcriptional regulators for cellular differentiation and growth. Klf2 activates transcription through its binding of the beta-globin gene promoter CACCC box. Studies have shown Klf2 to have critical roles in both the function and differentiation of several cell types including; adipocytes, lung cells, T lymphocytes, and monocytes. It has also been newly identified as an endothelial transcription regulator for pro-inflammatory activation, and is a requirement for the migration of smooth muscle cells and normal vessel formation. Shear stress and pro-inflammatory cytokines are considered to be potent inhibitors of Klf2. Expression is primarily observed in lung and spleen tissue, but it has also been found in aortic tissue.
Immunogen
Epitope: Unknown
GST-fusion protein with histidine-tagged mouse Klf2.
Application
Chromatin Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated Klf2 from 2 x 10E6 to 1 x 10E7 mouse embryonic stem cells. (Huck Ng, Genome Institute of Singapore.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-Klf2 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Klf2 also known as Kruppel-like factor 2 (lung), Lung krueppel-like factor.
Quality
Evaluated by Western Blot in mouse embryonic stem cell chromatin extract.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Klf2 in 10 µg of mouse embryonic stem cell chromatin extract.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Klf2 in 10 µg of mouse embryonic stem cell chromatin extract.
Target description
~40 kDa observed
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Mouse embryonic stem cell chromatin extract
Mouse embryonic stem cell chromatin extract
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Yuki Hirata et al.
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Klf2 is an essential factor that sustains ground state pluripotency.
Yeo, JC; Jiang, J; Tan, ZY; Yim, GR; Ng, JH; Goke, J; Kraus, P; Liang, H; Gonzales et al.
Cell Stem Cell null
Chenfeng Liu et al.
Science advances, 7(41), eabg6262-eabg6262 (2021-10-09)
Molecular pathways controlling emigration of mature thymocytes from thymus to the periphery remain incompletely understood. Here, we show that T cell–specific ablation of glycogen synthase kinase 3 (GSK3) led to severely impaired thymic egress. In the absence of GSK3, β-catenin
Dongbo Qiu et al.
Stem cell reports, 5(3), 314-322 (2015-09-01)
Activation of Wnt/β-catenin signaling can induce both self-renewal and differentiation in naive pluripotent embryonic stem cells (ESCs). To gain insights into the mechanism by which Wnt/β-catenin regulates ESC fate, we screened and characterized its downstream targets. Here, we show that
Amy F Chen et al.
Cell stem cell, 23(2), 226-238 (2018-07-19)
The enhancer landscape of pluripotent stem cells undergoes extensive reorganization during early mammalian development. The functions and mechanisms behind such reorganization, however, are unclear. Here, we show that the transcription factor GRHL2 is necessary and sufficient to activate an epithelial
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