All Photos(1)
APECED protein, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy
protein, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy 2
autoimmune regulator
, autoimmune regulator (APECED protein), autoimmune
protein, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy 2
autoimmune regulator
, autoimmune regulator (APECED protein), autoimmune
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Aire(11634)
General description
AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).
Specificity
Likely to cross-react with rat, based on sequence homology. Cross-reactivity with other species has not been tested
Recognizes murine AIRE, Mr 58 kDa (calculated). Appears at ~65 kDa in Western blots.
Immunogen
Epitope: a.a. 162-181
KLH-conjugated synthetic peptide corresponding to amino acids 162-181 of mouse AIRE
Application
Suggested dilutions:
Western blot: 1:1000
Western blot: 1:1000
Detect AIRE using this Anti-AIRE Antibody validated for use in WB.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Developmental Signaling
Transcription Factors
RNA Binding Protein (RBP)
Developmental Signaling
Transcription Factors
RNA Binding Protein (RBP)
Quality
Routinely evaluated by Western Blot analysis.
Western Blot:
A 1:1000 dilution of this lot detected AIRE in murine thymus lysate
Western Blot:
A 1:1000 dilution of this lot detected AIRE in murine thymus lysate
Target description
65 kDa
Physical form
100 μg of immunoaffinity purified rabbit polyclonal IgG in 173 μl of 0.02M PBS, 0.25M NaCl containing 0.1% sodium azide
ImmunoAffinity Purified
Storage and Stability
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analysis Note
Control
Murine thymus tissue lysate
Murine thymus tissue lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Journal of immunology (Baltimore, Md. : 1950), 174(4), 1862-1870 (2005-02-09)
Autoimmune regulator (AIRE) gene mutation is responsible for the development of organ-specific autoimmune disease with monogenic autosomal recessive inheritance. Although Aire has been considered to regulate the elimination of autoreactive T cells through transcriptional control of tissue-specific Ags in thymic
Science (New York, N.Y.), 298(5597), 1395-1401 (2002-10-12)
Humans expressing a defective form of the transcription factor AIRE (autoimmune regulator) develop multiorgan autoimmune disease. We used aire- deficient mice to test the hypothesis that this transcription factor regulates autoimmunity by promoting the ectopic expression of peripheral tissue- restricted
Immunity, 23(2), 227-239 (2005-08-23)
Aire promotes the tolerization of thymocytes by inducing the expression of a battery of peripheral-tissue antigens in thymic medullary epithelial cells. We demonstrate that the cellular mechanism by which Aire exerts its tolerance-promoting function is not primarily positive selection of
Human molecular genetics, 11(4), 397-409 (2002-02-21)
Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is a monogenic autosomal recessive disease caused by mutations in the AIRE gene. Here we have produced knock-out mice for the Aire gene. The Aire-/- mice develop normally; however, autoimmune features of APECED in Aire-/- mice
Nature immunology, 4(4), 350-354 (2003-03-04)
Autoimmune polyendocrinopathy syndrome type 1 is a recessive Mendelian disorder resulting from mutations in a novel gene, AIRE, and is characterized by a spectrum of organ-specific autoimmune diseases. It is not known what tolerance mechanisms are defective as a result
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service