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Merck
CN

08-126

Human Vitronectin

recombinant, expressed in E. coli, liquid, 1.5 mg/mL, suitable for cell culture

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

Key Documents

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Product Name

Vitronectin, human recombinant,

biological source

human

Quality Level

100

recombinant

expressed in E. coli

form

liquid

mol wt

Mw 78 kDa

manufacturer/tradename

Upstate®

concentration

1.5 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)

NCBI accession no.

NM_000638.3

UniProt accession no.

P04004

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... VTN(7448)

Related Categories

General description

Product Source: N-terminal, GST-tagged, full-length human Vitronectin expressed in E. coli

Physical form

Liquid in 10mM Sodium Phosphate, pH 7.1, 150mM Sodium Chloride, 0.1mM DTT, 0.1mM PMSF and 50% glycerol.

Preparation Note

6 months at -20°C from date of shipment

Analysis Note

Routinely evaluated by cell adhesion assay.

Other Notes

Replaces: CC080

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

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Expression, production, and characterization of full-length vitronectin in Escherichia coli
Wojciechowski, Katherine, et al
Protein Expression and Purification, 36, 131-138 (2004)
I Schvartz et al.
The international journal of biochemistry & cell biology, 31(5), 539-544 (1999-07-10)
Vitronectin is a multifunctional glycoprotein present in blood and in the extracellular matrix. It binds glycosaminoglycans, collagen, plasminogen and the urokinase-receptor, and also stabilizes the inhibitory conformation of plasminogen activation inhibitor-1. By its localization in the extracellular matrix and its
R J Wechsler-Reya
Trends in neurosciences, 24(12), 680-682 (2001-11-24)
Cerebellar granule cells are the most abundant neurons in the brain and are crucial to the circuitry that controls motor coordination. The proliferation of granule cell precursors (GCPs) is controlled by the secreted signaling molecule Sonic hedgehog (Shh), but the

Protocols

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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