Anti-α-Insulin Receptor Antibody, β subunit

The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.

This antibody recognizes α-Insulin Receptor, β subunit.

Epitope: C-terminus

GST-fusion protein corresponding to the last 100 C-terminal amino acid residues of the human insulin receptor β chain

Anti-α-Insulin Receptor Antibody, β subunit is an antibody against a-Insulin Receptor for use in IP & WB.

Immunoprecipitation Analysis:

Research Category
Signaling

Metabolism

Research Sub Category
Insulin/Energy Signaling

Evaluated by Western Blot in Human brain tissue lysate.
Western Blot Analysis: : 1:1,000 dilution of this antibody detected α-Insulin Receptor, β subunit in human brain tissue lysate.

97 kDa

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

Maintain refrigerated at 2-8°C for for 1 year from date of receipt.

Control
Human brain tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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