All Photos(2)
Synonym(s):
BIG3, BMP2-induced 3-kb gene protein, Cilia and flagella associated protein 89, Set1c WD40 repeat protein homolog, SWD3, WD repeat-containing protein 5, WD-repeat protein 5
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... WDR5(11091)
General description
WD repeat-containing protein 5 (also known as BMP2-induced 3-kb gene protein, WD repeat-containing protein 5, WD-repeat protein 5, Set1c WD40 repeat protein homolog, cilia and flagella associated protein 89) is a nuclear protein encoded by the WDR5 (also known as BIG3, CFAP89, SWD3) gene in human. This protein contains 7 WD (Trp-Asp) repeats and belongs to the WD repeat protein family whose members are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WD repeat-containing protein 5 is a component of several complexes that regulate histone methylation and acetylation levels, including ATAC (ADA2A-containing complex) complex, SET1 complex, MLL1/MLL, MLL2/3 (also named ASCOM complex), MLL4/WBP7, and NSL (non-specific lethal) complex.
Specificity
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.
Recognizes WDR5, MW 36 kDa. A related protein band of 43 kDa that was blocked by the immunizing peptide was also detected. Identification of WDR5 protein at 36 kDa was confirmed with siRNA knockdown by an independent laboratory.
Immunogen
Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human WDR5.
Application
Detect WDR5 using this Anti-WDR5 Antibody validated for use in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blot: 0.05-0.25 μg/mL using HeLa nuclear extracts.
Quality
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.
Target description
~39 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Yul W Yang et al.
eLife, 3, e02046-e02046 (2014-02-14)
The WDR5 subunit of the MLL complex enforces active chromatin and can bind RNA; the relationship between these two activities is unclear. Here we identify a RNA binding pocket on WDR5, and discover a WDR5 mutant (F266A) that selectively abrogates
Molecular regulation of H3K4 trimethylation by Wdr82, a component of human Set1/COMPASS.
Wu, M; Wang, PF; Lee, JS; Martin-Brown, S; Florens, L; Washburn, M; Shilatifard, A
Molecular and cellular biology null
B Bidon et al.
Nature communications, 9(1), 2610-2610 (2018-07-06)
The DNA damage sensor XPC is involved in nucleotide excision repair. Here we show that in the absence of damage, XPC co-localizes with RNA polymerase II (Pol II) and active post-translational histone modifications marks on a subset of class II
Jae-Hwan Kim et al.
Cancer research, 74(6), 1705-1717 (2014-02-05)
Histone methyltransferases and demethylases reversibly modulate histone lysine methylation, which is considered a key epigenetic mark associated with gene regulation. Recently, aberrant regulation of gene expression by histone methylation modifiers has emerged as an important mechanism for tumorigenesis. However, it
J Paggetti et al.
Oncogene, 29(36), 5019-5031 (2010-06-29)
MOZ and MLL, encoding a histone acetyltransferase (HAT) and a histone methyltransferase, respectively, are targets for recurrent chromosomal translocations found in acute myeloblastic or lymphoblastic leukemia. In MOZ (MOnocytic leukemia Zinc-finger protein)/CBP- or mixed lineage leukemia (MLL)-rearranged leukemias, abnormal levels
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