Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor^™ 647 Conjugate

Histone H3 is one of the five main histone proteins that are involved in the structure of chromatin in eukaryotic cells. Histone H3 features a main globular domain and a long N-terminal tail. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 is specifically phosphorylated during both mitosis and meiosis in patterns that are specifically coordinated in space and time. These phosphorylations are initiated at different phases of the cell division. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Expected to react with most other species based on extreme conserved homology among species.

Epitope: Trimethylated (Lys4)

Synthetic peptide corresponding to residues surrounding and including trimethlyated Lys 4 of Histone H3.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in NIH/3T3 cells.
The unconjugated version of this antibody has been shown to work in ChIP, DB, WB, ChIP-seq.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor^™ 647 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys4) in A431 cells.

~16 kDa observed

Affinity purified

Purified rabbit conjugate antibody in buffer containing PBS with 15 mg/ml BSA and 0.1% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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