07-473-AF488
Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor™ 488 Conjugate
from rabbit, ALEXA FLUOR™ 488
Sign Into View Organizational & Contract Pricing
All Photos(2)
H3K4me3, Histone H3 (trimethyl Lys4), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
Recommended Products
biological source
rabbit
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
vertebrates (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
trimethylation (Lys4 )
Gene Information
human ... H3C1(8350)
mouse ... H3C1(360198)
General description
Histone H3 is one of the five main histone proteins that are involved in the structure of chromatin in eukaryotic cells. Histone H3 features a main globular domain and a long N-terminal tail. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 is specifically phosphorylated during both mitosis and meiosis in patterns that are specifically coordinated in space and time. These phosphorylations are initiated at different phases of the cell division. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
Specificity
Expected to react with most other species based on extreme conserved homology among species.
Immunogen
Epitope: Trimethylated (Lys4)
Synthetic peptide corresponding to residues surrounding and including trimethlyated Lys 4 of Histone H3.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in A431 and NIH/3T3 cells.
The unconjugated version of this antibody has been shown to work in ChIP, DB, WB, ChIP-seq.
The unconjugated version of this antibody has been shown to work in ChIP, DB, WB, ChIP-seq.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor™ 488 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.
Quality
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys4) in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys4) in HeLa cells.
Target description
~16 kDa observed
Physical form
Affinity purified
Purified rabbit conjugate antibody in buffer containing PBS with 15 mg/ml BSA and 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service