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07-441-AF488

Sigma-Aldrich

Anti-dimethyl-Histone H3 (Lys9) Antibody, Alexa Fluor™ 488 Conjugate

from rabbit, ALEXA FLUOR™ 488

Synonym(s):

Histone H3.1t, H3/t, H3t, H3/g, dimethyl-Histone H3 (Lys9), AlexaFluor™ 488 Conjugate

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), yeast (based on 100% sequence homology), chicken (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

dimethylation (Lys9)

Gene Information

human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Specificity

Histone H3 dimethylated on lysine 9.

Immunogen

Epitope: H3K9me2
KLH-conjugated, 2X-branched synthetic peptide containing the sequence ..RKme2STG.., in which Kme2 corresponds to dimethyl-lysine at residue 9 of human Histone H3.

Application

Anti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor 488 Conjugate is an antibody against dimethyl-Histone H3 (Lys9) Antibody for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl-Histone H3 (Lys9) in HepG2 cells.
The unconjugated antibody (Cat. No. 07-441) is shown to be suitable also for Western blotting, dot blot, immunocytochemistry, and peptide inhibition assay (peptide blocking) applications.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected dimethyl-Histone H3 (Lys9) in HeLa cells.

Target description

~17 kDa observed

Physical form

Protein A purified
Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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