All Photos(2)
Synonym(s):
S100 calcium binding protein A4, S100 calcium-binding protein A4, fibroblast-specific protein-1, leukemia multidrug resistance associated protein, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), malig
UNSPSC Code:
12352203
eCl@ss:
32160702
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biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... S100A4(6275)
mouse ... S100A4(20198)
General description
Fibroblast-specific protein-1 (FSP1)/S100A4 is a cytoplasmic calcium-binding protein belonging to the S100 superfamily of which at least 21 members have been identified so far. Researchers believe that FSP1/S1004A is highly expressed in fibroblasts (Iwano et al., 2001; Strutz et al., 1995), in epithelial and endothelial cells undergoing epithelial-mesenchymal transition (EMT) (Iwano et al., 2002; Zeisberg and Neilson, 2009), and epithelial cancer cells that complete a transition to a metastatic phenotype (Xue et al., 2003).
Immunogen
Epitope: Unknown
Histidine-tagged recombinant protein corresponding to mouse FSP1/S100-A4.
Application
Detect the FSP1/S100A4 protein using this Anti-FSP1/S100A4 validated for use in WB & IHC.
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Western Blot Analysis: 1 µg/mL from a previous lot detected S100-A4 on 10 µg of HeLa cell lysate.
Quality
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected FSP1/S100-A4 on 10 µg of NIH/3T3 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected FSP1/S100-A4 on 10 µg of NIH/3T3 cell lysate.
Target description
~ 12 kDa
Physical form
Format: Purified
Protein G Purified
Purified rabbit polyclonal containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
NIH/3T3 cell lysate
NIH/3T3 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Thomas Bärnthaler et al.
The Journal of allergy and clinical immunology, 145(3), 818-833 (2019-12-10)
Idiopathic pulmonary fibrosis (IPF) is a disease with high 5-year mortality and few therapeutic options. Prostaglandin (PG) E2 exhibits antifibrotic properties and is reduced in bronchoalveolar lavage from patients with IPF. 15-Prostaglandin dehydrogenase (15-PGDH) is the key enzyme in PGE2
Tristan Lerbs et al.
JCI insight, 5(16) (2020-08-21)
Scleroderma is a devastating fibrotic autoimmune disease. Current treatments are partly effective in preventing disease progression but do not remove fibrotic tissue. Here, we evaluated whether scleroderma fibroblasts take advantage of the "don't-eat-me-signal" CD47 and whether blocking CD47 enables the
Elizabeth F Redente et al.
JCI insight, 6(1) (2020-12-09)
Idiopathic pulmonary fibrosis (IPF) is a progressive, irreversible fibrotic disease of the distal lung alveoli that culminates in respiratory failure and reduced lifespan. Unlike normal lung repair in response to injury, IPF is associated with the accumulation and persistence of
Xinyi Wang et al.
JCI insight, 5(12) (2020-05-13)
Renal fibrosis features exaggerated inflammation, extracellular matrix (ECM) deposition, and peritubular capillary loss. We previously showed that IL-10 stimulates high-molecular weight hyaluronan (HMW-HA) expression by fibroblasts, and we hypothesize that HMW-HA attenuates renal fibrosis by reducing inflammation and ECM remodeling.
Meiling Melzer et al.
Journal of visualized experiments : JoVE, (157) (2020-04-01)
Cardiac fibrosis in response to injury is a physiological response to wound healing. Efforts have been made to study and target fibroblast subtypes that mitigate fibrosis. However, fibroblast research has been hindered due to the lack of universally acceptable fibroblast
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