Anti-Cysteine Sulfenic Acid Antibody

Protein sulfenic acid formation is a reversible post-translational modification that may be used to monitor protein oxidation on reactive cysteines within target proteins. This can be detected with protein sulfenic acid derivatised with dimedone.

This antibody recognizes proteins containing cysteine sulfenic acid derivitized with dimedone.

Epitope: Other

KLH-conjugated corresponding to cysteine sulfenic acid.

Anti-Cysteine Sulfenic Acid detects levels of Cysteine Sulfenic Acid proteins & has been published & validated for use in WB.

Research Category
Apoptosis & Cancer

Research Sub Category
General Post-translation Modification

Western Blot Analysis: A previous lot of this antibody detected cysteine sulfenic acid in NIH/3T3 and HEK293 cell lysates.

Evaluated by Western Blot in rat ventricular myocyte lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected cysteine sulfenic acid on 10 µg of rat ventricular myocyte lysate.

Multiple kDa are observed that increases with oxidized stress (H2O2).

Unpurified

Unpurified rabbit polyclonal with 0.05% sodium azide.

Stable for 1 year at from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Rat ventricular myocyte lysate

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