All Photos(4)
Synonym(s):
phosphatidylethanolamine N-methyltransferase
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PEMT(10400)
General description
PEMT (phosphatidylethanolamine N-methyltransferase) is an an important enzyme for the synthesis of membrane phospholipids. PEMT converts phosphatidylethanolamine to phosphatidyl-choline by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Inhibition of hepatic PEMT activity has been associated with Alzheimer’s disease and schizophrenia.
Specificity
Other homologies: Mouse (86% sequence homology) and Rat (73% sequence homology).
This antibody recognizes the C-terminal region of human PEMT in Jurkat cell lysates. The antibody easily detected PEMT in Jurkat and L6 lysates by Western blotting, but expression was weak or not detected in A431, HeLa, HEK293, PC12, or NIH/3T3 cell lysates using very dilute antibody (0.25 μg/mL, data not shown). Positive signals in immunocytochemistry using higher concentrations of antibody (2.5 μg/mL) detected PEMT in these cell lines, suggesting differential expression levels of this protein in various cell lines.
Immunogen
Epitope: C-terminus
The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PEMT.
Application
ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA
Immunocytochemistry:
HeLa, A431, and NIH/3T3 (previous page) cells incubated with anti-PEMT (C-terminus) stained positively on nuclei and endoplasmic reticulum (ER).
This antibody was reported by an outside laboratory to be suitable for ELISA
Immunocytochemistry:
HeLa, A431, and NIH/3T3 (previous page) cells incubated with anti-PEMT (C-terminus) stained positively on nuclei and endoplasmic reticulum (ER).
Research Category
Epigenetics & Nuclear Function
Neuroscience
Epigenetics & Nuclear Function
Neuroscience
Research Sub Category
Chromatin Biology
Chromatin Biology
This PEMT antibody is validated for use in WB, ICC & ELISA for the detection of the PEMT protein.
Quality
Western Blot Analysis:
1:1,000 dilution of this antibody detected PEMT on 10 µg of L6 lysate (below) and Jurkat lysate (not shown).
1:1,000 dilution of this antibody detected PEMT on 10 µg of L6 lysate (below) and Jurkat lysate (not shown).
Target description
~22 kDa
Physical form
Format: Purified
Protein A chromatography
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage and Stability
Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
Analysis Note
Control
L6 and Jurkat lysates (Western blotting), HeLa, A431 cells (Immunocytochemistry)
L6 and Jurkat lysates (Western blotting), HeLa, A431 cells (Immunocytochemistry)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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C J Walkey et al.
Biochimica et biophysica acta, 1436(3), 405-412 (1999-02-16)
Phosphatidylethanolamine is converted to phosphatidylcholine in mammalian liver by the enzyme phosphatidylethanolamine N-methyltransferase (PEMT). A form of the enzyme (PEMT2) has been isolated from rat liver, the cDNA cloned and expressed and the murine gene has been characterized and disrupted.
D J Shields et al.
Biochimica et biophysica acta, 1532(1-2), 105-114 (2001-06-23)
Phosphatidylethanolamine N-methyltransferase (PEMT) catalyzes the conversion of phosphatidylethanolamine (PE) to phosphatidylcholine (PC) in a series of three methylation reactions. Preliminary studies of PEMT in humans led to the cloning of three cDNAs each of which has a different 5' untranslated
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