All Photos(1)
Synonym(s):
SIR2-like protein 3 , mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SIRT3(23410)
mouse ... Sirt3(64384)
General description
SIRT3 is a member of the class I sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT3 is located in the mitochondria and has been implicated in regulating metabolic processes. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of Sirt3 in cultured cells increases respiration and decreases the production of reactive oxygen species. Fasting increases Sirt3 expression in white and brown adipose tissue. A longer form of SIRT3 is present in the nucleus where it exhibits histone deacetylase activity. SIRT3 protects cardiomyocytes from stress mediated cell death due to deacetylation of a nuclear factor, Ku-70.
Specificity
Not tested in other species.
This antibody recognizes SIRT3. Endogenous levels of this protein vary among cell lines; the protein was easily detected in RAW264.7 and C2C12 cells but only weakly detected in HeLa cells, and not detected in Jurkat, HEK293 or PC3 cells.
Immunogen
Epitope: C-terminus
The immunogen was a peptide corresponding the C-terminal region of human SIRT3.
Application
Anti-SIRT3 (C-terminus) Antibody is a Rabbit Polyclonal Antibody for detection of SIRT3 (C-terminus) also known sirtuin 3 & has been validated in WB, ELISA & IHC.
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).
Immunohistochemistry:
This antibody has been shown by an outside laboratory to be suitable for histochemistry using human hepatocarcinoma tissue (1:50 – 1:100).
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).
Immunohistochemistry:
This antibody has been shown by an outside laboratory to be suitable for histochemistry using human hepatocarcinoma tissue (1:50 – 1:100).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Histone Modifying Proteins
Quality
Western Blot Analysis:
1:250 dilution of this antibody detected SIRT3 on 10 µg of C2C12 lysate.
1:250 dilution of this antibody detected SIRT3 on 10 µg of C2C12 lysate.
Target description
44 kDa
Physical form
Format: Purified
Purified
Purified rabbit polyclonal in buffer containing 0.09% NaN3.
Storage and Stability
Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
C2C12 cell lysate
C2C12 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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