07-1585
Anti-HIF-1-alpha Antibody, hydroxyproline (Pro402)
from rabbit, purified by affinity chromatography
Synonym(s):
Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... HIF1A(3091)
General description
Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with HIF-1β to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.
Specificity
This antibody recognizes HIF-1-alpha at the ODD region.
Immunogen
Epitope: ODD region
KLH-conjugated linear peptide corresponding to the ODD region of human HIF-1-alpha.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-HIF-1-alpha Antibody, hydroxyproline (Pro402) (Rabbit Polyclonal Antibody) validated in WB to detect HIF-1-alpha also known as Hypoxia-inducible factor 1-alpha, HIF1-alpha.
Quality
Evaluated by Western Blot in FeCl2 treated HIF-1-alpha recombinant proteins.
Western Blot Analysis: 1 µg/mL of this antibody detected HIF-1-alpha on 10 µg of FeCl2 treated HIF-1-alpha recombinant proteins.
Western Blot Analysis: 1 µg/mL of this antibody detected HIF-1-alpha on 10 µg of FeCl2 treated HIF-1-alpha recombinant proteins.
Target description
~120 kDa observed using Gal4-tagged recombinant protein. 92 kDa calculated for native protein.
Linkage
Replaces: 04-1006
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
FeCl2 treated HIF-1-alpha recombinant proteins
FeCl2 treated HIF-1-alpha recombinant proteins
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Ya-Min Tian et al.
The Journal of biological chemistry, 286(15), 13041-13051 (2011-02-22)
Hypoxia inducible factor (HIF) is regulated by dual pathways involving oxygen-dependent prolyl and asparaginyl hydroxylation of its α-subunits. Prolyl hydroxylation at two sites within a central degradation domain promotes association of HIF-α with the von Hippel-Lindau ubiquitin E3 ligase and
Michael I Koukourakis et al.
Scientific reports, 6, 30986-30986 (2016-08-11)
The mechanism of Amifostine (WR-2721) mediated radioprotection is poorly understood. The effects of amifostine on human basal metabolism, mouse liver metabolism and on normal and tumor hepatic cells were studied. Indirect calorimetric canopy tests showed significant reductions in oxygen consumption
T E McAllister et al.
Chemical science, 9(20), 4569-4578 (2018-06-15)
Affinity reagents are of central importance for selectively identifying proteins and investigating their interactions. We report on the development and use of cyclic peptides, identified by mRNA display-based RaPID methodology, that are selective for, and tight binders of, the human
Proline-hydroxylated hypoxia-inducible factor 1? (HIF-1?) upregulation in human tumours.
Snell, CE; Turley, H; McIntyre, A; Li, D; Masiero, M; Schofield, CJ; Gatter, KC; Harris et al.
Testing null
Linda O'Flaherty et al.
Human molecular genetics, 19(19), 3844-3851 (2010-07-28)
Mutations in the gene encoding the Krebs cycle enzyme fumarate hydratase (FH) predispose to hereditary leiomyomatosis and renal cell cancer in affected individuals. FH-associated neoplasia is characterized by defective mitochondrial function and by upregulation of transcriptional pathways mediated by hypoxia-inducible
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service