07-1464
Anti-Rac1 Antibody
from rabbit
Synonym(s):
Ras-related C3 Botulinum Toxin Substrate 1, p21-Rac1, Ras-like Protein TC25
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, mouse
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RAC1(5879)
General description
Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac.
Specificity
Recognizes Rac1, does not cross react with Cdc42. Rac1 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Rac regulates actin polymerization to form lamellipodia.
Immunogen
Synthetic peptide corresponding to amino acids 130-141 of human Rac1 (KEKKLTPITYPQ-C).
Application
Anti-Rac1 Antibody is an antibody against Rac1 for use in WB.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Quality
Routinely evaluated by Western Blot on L6 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected RAC1 on 10 μg of L6 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected RAC1 on 10 μg of L6 lysates.
Target description
21 kDa
Linkage
Replaces: AB4202
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative
Storage and Stability
Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections
Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Jeffrey M Hord et al.
Experimental gerontology, 83, 148-157 (2016-08-19)
Age-related loss of skeletal muscle mass and function, referred to as sarcopenia, is mitigated by lifelong calorie restriction as well as exercise. In aged skeletal muscle fibers there is compromised integrity of the cell membrane that may contribute to sarcopenia.
Joon-Il Jun et al.
Nature cell biology, 12(7), 676-685 (2010-06-08)
Cellular senescence is a recognized mechanism of tumour suppression; however, its contribution to other pathologies is not well understood. We show that the matricellular protein CCN1 (also known as CYR61; cysteine-rich protein 61), which is dynamically expressed at sites of
D Araiza-Olivera et al.
Oncogene, 37(7), 944-952 (2017-10-24)
Activating mutations in the RAC1 gene have recently been discovered as driver events in malignant melanoma. Expression of this gene is associated with melanocyte proliferation, and melanoma cells bearing this mutation are insensitive to BRAF inhibitors such as vemurafenib and
Nicolas Bery et al.
Cell chemical biology, 26(11), 1544-1558 (2019-09-17)
The selective downregulation of activated intracellular proteins is a key challenge in cell biology. RHO small GTPases switch between a guanosine diphosphate (GDP)-bound and a guanosine triphosphate (GTP)-bound state that drives downstream signaling. At present, no tool is available to
Jeffrey M Hord et al.
Physiological reports, 9(1), e14606-e14606 (2021-01-06)
Reduced mechanical loading results in atrophy of skeletal muscle fibers. Increased reactive oxygen species (ROS) are causal in sarcolemmal dislocation of nNOS and FoxO3a activation. The Nox2 isoform of NADPH oxidase and mitochondria release ROS during disuse in skeletal muscle.
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