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Merck
CN

07-1341

Anti-PHLPP1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

PH domain and leucine rich repeat protein phosphatase, PH domain leucine-rich repeat protein phosphatase, Pleckstrin homology domain-containing family E protein 1, SCN circadian oscillatory protein, Suprachiasmatic nucleus circadian oscillatory protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-PHLPP1 Antibody, from rabbit, purified by affinity chromatography

biological source

rabbit

conjugate

conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

purified by

affinity chromatography

species reactivity

rat, mouse, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

immunogen sequence

KLH-conjugated linear peptide corresponding to the C-terminus of PHLPP1.

NCBI accession no.

NP_919431

UniProt accession no.

O60346

shipped in

wet ice

storage temp.

2-8°C

Quality Level

100

Gene Information

human ... PHLPP1(23239)

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of PHLPP1.
Epitope: C-terminus

General description

PHLPP1 is the protein phosphatase responsible for dephosphorylation of the Akt (Ser473) site. Akt (Ser473) is the TORC2 (mTOR) phosphorylation activation site.
134 kDa Observed at 110 kDa

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Application

Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
Anti-PHLPP1 Antibody detects level of PHLPP1 & has been published & validated for use in WB & IC.

Biochem/physiol Actions

This antibody recognizes the C-terminal region of PHLPP1.

Analysis Note

Control
Human brain tissue lysate
Evaluated by Western Blot in Human brain tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected PHLPP1 in Human brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified
Purified rabbit serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Saida Abdelli et al.
PloS one, 7(5), e35997-e35997 (2012-05-09)
We have recently shown that silencing of the brain/islet specific c-Jun N-terminal Kinase3 (JNK3) isoform enhances both basal and cytokine-induced beta-cell apoptosis, whereas silencing of JNK1 or JNK2 has opposite effects. While it is known that JNK1 or JNK2 may
Travis C Jackson et al.
Scientific reports, 5, 9377-9377 (2015-04-02)
Pleckstrin homology domain and leucine rich repeat protein phosphatase 1 (PHLPP1) is a member of the serine/threonine family of phosphatases. It has been studied in organs including brain, heart, pancreas, adipose, breast, and prostate. Human PHLPP1 encodes two splice variants
Georgia Balsevich et al.
Nature communications, 8(1), 1725-1725 (2017-11-25)
The co-chaperone FKBP5 is a stress-responsive protein-regulating stress reactivity, and its genetic variants are associated with T2D related traits and other stress-related disorders. Here we show that FKBP51 plays a role in energy and glucose homeostasis. Fkbp5 knockout (51KO) mice
Clara M Castillejo Becerra et al.
Journal of cellular biochemistry, 119(9), 7470-7478 (2018-05-19)
The protein phosphatase Phlpp1 is an essential enzyme for proper chondrocyte function. Altered Phlpp1 levels are associated with cancer and degenerative diseases such as osteoarthritis. While much is known about the post-transcriptional mechanisms controlling Phlpp1 levels, transcriptional regulation of the
Ismael Y Karkache et al.
JBMR plus, 7(12), e10806-e10806 (2023-12-22)
Prior work demonstrated that Phlpp1 deficiency alters limb length and bone mass, but the cell types involved and requirement of Phlpp1 for this effect were unclear. To understand the function of Phlpp1 within bone-forming osteoblasts, we crossed Phlpp1 floxed mice
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