06-756
Anti-Frizzled 5 Antibody
Upstate®, from rabbit
Synonym(s):
Fz5, Fzd5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FZD5(7855)
General description
The Wnt family consists of ligands involved in cell fate determination, organogenesis, and oncogenesis. The Wnt signal is thought to be transduced sequentially via a homolog of the frizzled family of receptors to GSK-3 (disheveled), β- and γ- catenin and finally to members of the Tcf/Lef-1 family of HMG box transcription factors. Frizzled 5 is believed to function as the receptor for the Wnt5a ligand.
Specificity
Frizzled 5 (aka Fz5 or Fzd5)
Immunogen
Epitope: C-Terminus
KLH-conjugated, synthetic peptide derived from amino acids 564-585 of human Frizzled 5 (CTGRTPPGPAATYHKQVSLSHV).
Application
Anti-Frizzled 5 Antibody is an antibody against Frizzled 5 for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Developmental Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Quality
Routinely evaluated by immunoblot on lysates from mouse liver, C2C12, Jurkat or HUVEC cells
Target description
Frizzled 5 (62kDa)
Physical form
Format: Purified
Protein A chromatography
Purified antibody in PBS with 0.05% sodium azide and 30% Glycerol
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Lysates from mouse liver, C2C12, Jurkat, and HUVEC
Lysates from mouse liver, C2C12, Jurkat, and HUVEC
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Wnt/beta-catenin signaling pathway as a novel cancer drug target.
Luu, Hue H, et al.
Current Cancer Drug Targets, 4, 653-671 (2004)
Norrin and frizzled; a new vein for the eye.
Niehrs, Christof
Developmental Cell, 6, 453-454 (2004)
X He et al.
Science (New York, N.Y.), 275(5306), 1652-1654 (1997-03-14)
In Xenopus laevis embryos, the Wingless/Wnt-1 subclass of Wnt molecules induces axis duplication, whereas the Wnt-5A subclass does not. This difference could be explained by distinct signal transduction pathways or by a lack of one or more Wnt-5A receptors during
P Bhanot et al.
Nature, 382(6588), 225-230 (1996-07-18)
Receptors for Wingless and other signalling molecules of the Wnt gene family have yet to be identified. We show here that cultured Drosophila cells transfected with a novel member of the frizzled gene family in Drosophila, Dfz2, respond to added
WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins.
Michaela Bc Kilander,Jacomijn P Dijksterhuis,Ranjani Sri Ganji,Vitezslav Bryja,Gunnar Schulte
Cellular Signalling null
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