Anti-FcεRI Antibody, γ subunit

High affinity immunoglobulin epsilon receptor subunit gamma (UniProt P20411; also known as Fc-epsilon RI-gamma, Fc receptor gamma-chain, FceRI gamma, FcR gamma, IgE Fc receptor subunit gamma) is encoded by the Fcer1g (also known as Fce1g) gene (Gene ID: 25441) in rat species. FceR1 gamma is a single-pass type I membrane protein that associates with a variety of FcR alpha chains to form a functional signaling complex that regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface. FceRI gamma is found exclusively on mast cells and basophils. In rodents Fc epsilon RI is a tetrameric complex of non-covalently attached subunits: one IgE-binding alpha subunit, one beta subunit and a dimer of disulfide-linked gamma subunits. When multivalent allergens bind to the receptor-bound IgE, the consequent aggregation of the receptors leads to the release of mediators responsible for allergic symptoms. (Ref.: Blank, U., et al. (1989). Nature 337(6203); 187-189).

Other species cross-reactivity not tested.

Recognizes and is specific for FcεRI, γ-subunit, MW 10kDa.

KLH-conjugated linear peptide corresponding to 7 amino acids from the C-terminal region of rat FceRI gamma subunit.

Anti-FceRI, gamma subunit, Cat. No. 06-727, is a rabbit polyclonal antibody that detects FceRI, subunit and has been tested for use in Flow Cytometry and Western Blotting.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected FceRI, gamma subunit in rat basophilic leukemia cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Evaluated by western blot on RIPA lysate of RBL cells.

Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FcεRI, γ-subunit in RIPA lysate of RBL cells.

30 kDa

Format: Purified

Protein A purified

Purified rabbit polylclonal IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol.

Stable for 1 year at from date of receipt.

Control
RBL cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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