Anti-Androgen Receptor Antibody, Alexa Fluor^™ 647 Conjugate

Androgen receptor (UniProt P15207; also known as Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4) is encoded by the Ar (also known as Andr, Nr3c4, Tfm) gene (Gene ID 24208) in rat species. Androgen receptor (AR) is a hormone-activated transcription regulator that plays important roles in normal prostate development and function, as well as in malignant prostate transformation. The binding of an androgenic hormone (testosterone or dihydrotestosterone) induces a conformational change in AR, resulting its dissociation from heat schock proteins and subsequent translocation from the cytosol to nucleus, where it dimerizes and mediates either transcription activation or repression in a target gene-specific manner. The androgen insensitivity syndrome (AIS) or testicular feminization (TFM) is caused by a mutation of the androgen receptor gene located on the X chromosome.

Epitope: This antibody recognizes the Modulating Region within the N-terminal of androgen receptor.

KLH-conjugated linear peptide corresponding to the N-terminal sequence of rat androgen receptor.

Detect Androgen Receptor using this Anti-Androgen Receptor Antibody, Alexa Fluor^™ 647 Conjugate validated for use in Immunocytochemistry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Hormones & Receptors

The unconjugated antibody (Cat. No. 06-680) is shown to be suitable also for chromatin immunoprecipitation (ChIP), ChIP-seq, Western blotting, immunoprecipitation, flow cytometry, immunohistochemistry applications.

Evaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Androgen Receptor in MCF-7 cells.

~110 kDa observed.

Affinity purified

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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