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biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
clone
polyclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Immunogen
GST (Glutathione-S-transferase) derived from the pGEX expression vector and linked to KLH
Application
Anti-GST Tag Antibody detects level of GST Tag & has been published & validated for use in WB.
Quality
routinely evaluated by immunoblot on MAP Kinase 2/Erk2, active (Catalog # 14-173). 0.5-2mg/ml of a previous lot detected 500ng of GST (Catalog # 12-350)
Target description
28-30 kDa
Physical form
Format: Purified
Analysis Note
Control
Cells expressing the GST-tag
Cells expressing the GST-tag
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Laura Megrelis et al.
Frontiers in immunology, 9, 2001-2001 (2018-09-27)
We previously identified Fam65b as an atypical inhibitor of the small G protein RhoA. Using a conditional model of a Fam65b-deficient mouse, we first show that Fam65b restricts spontaneous RhoA activation in resting T lymphocytes and regulates intranodal T cell
Regulation of nuclear import/export of carbohydrate response element-binding protein (ChREBP): interaction of an alpha-helix of ChREBP with the 14-3-3 proteins and regulation by phosphorylation.
Sakiyama, H; Wynn, RM; Lee, WR; Fukasawa, M; Mizuguchi, H; Gardner, KH; Repa, JJ; Uyeda, K
The Journal of Biological Chemistry null
The telomeric protein TRF2 binds the ATM kinase and can inhibit the ATM-dependent DNA damage response.
Karlseder, J; Hoke, K; Mirzoeva, OK; Bakkenist, C; Kastan, MB; Petrini, JH; de Lange, T
PLoS Biology null
Lotus japonicus E3 ligase SEVEN IN ABSENTIA4 destabilizes the symbiosis receptor-like kinase SYMRK and negatively regulates rhizobial infection.
Den Herder, G; Yoshida, S; Antolin-Llovera, M; Ried, MK; Parniske, M
Plant Cell null
Andrea Brenna et al.
eLife, 8 (2019-11-07)
Circadian oscillations emerge from transcriptional and post-translational feedback loops. An important step in generating rhythmicity is the translocation of clock components into the nucleus, which is regulated in many cases by kinases. In mammals, the kinase promoting the nuclear import
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